Sodium chloride is added to maintain the osmotic equilibrium and agar is the solidifying agent. Usually, an incubation temperature of 35 ± 2° C is adequate. The combination of casein and soy peptones renders the medium nutritious by supplying organic nitrogen, particularly amino acids and longer-chained peptides. Trypticase soy agar or tryptone soya agar (TSA) and Trypticase soy broth or tryptone soya broth (TSB) with agar are growth media for the culturing of bacteria.They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow. This media provides all the basic components that are required by the bacterial cell for the rapid growth in the laboratory. Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic bacteria.It is a complex, general purpose medium that is routinely used to grow certain pathogenic bacteria, which tend to have high nutritional requirements (i.e., they are fastidious). For use as a suspension medium, inoculate the tube with a small amount of growth Incubate for 18 to 24 h or longer if required. The universal culture medium used in the microbiology laboratories that supports the cultivations of almost all types of bacteria is the Trypticase Soy Agar (TSA) and the Trypticase Soy Broth Medium (TSB), also known as the Tryptic Soy Broth or Tryptone Soya Broth. Preparation TSB Medium Preparation Columbia Agar with 5% Sheep Blood or BD Trypticase Soy Agar II with 5% Sheep Blood and, eventually, on additional selective and nonselective media. Tryptic Soy Agar contains digests of casein and soybean meal. Tryptic Soy Agar for microbiology, NutriSelect™ Plus; Synonym: CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soya Agar; find Millipore-22091 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.

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